Abstract
The formation of blood vessel networks is a fundamental event in vertebrate embryo development. Angiogenesis and vasculogenesis are the essential processes in vascular formation. Endothelial cells play a key role during angiogenesis and vasculogenesis, and cultured vascular endothelial cells provide an indispensable model for exploring the molecular mechanisms of angiogenesis and vasculogenesis. In this chapter, we described a protocol using PECAM-1-coated Dynabeads for the isolation of vascular endothelial cells from mouse heart and lung. This method will provide up to 107 endothelial cells with high purity (>85%). The isolated endothelial cells retain their in vivo characteristics, such as the expression of the cell surface markers PECAM-1 and ICAM-2.
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Acknowledgments
This work was supported by American Heart Association Scientist Development Grant and Texas A&M Health Science Center Startup Grant to Xu Peng.
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Jin, Y., Liu, Y., Antonyak, M., Peng, X. (2012). Isolation and Characterization of Vascular Endothelial Cells from Murine Heart and Lung. In: Peng, X., Antonyak, M. (eds) Cardiovascular Development. Methods in Molecular Biology, vol 843. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-61779-523-7_14
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DOI: https://doi.org/10.1007/978-1-61779-523-7_14
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Publisher Name: Humana Press, Totowa, NJ
Print ISBN: 978-1-61779-522-0
Online ISBN: 978-1-61779-523-7
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