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Wnt/Planar Cell Polarity Signaling in the Regulation of Convergent Extension Movements During Xenopus Gastrulation

  • Gun-Hwa KimEmail author
  • Edmond Changkyun Park
  • Jin-Kwan Han
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 839)

Abstract

The Wnt/planar cell polarity (PCP) signaling pathway plays a critical role in wing, eye, neural tube defects, and sensory bristle development of Drosophila and vertebrate development. Recently, the Wnt/PCP pathway has been known to regulate convergent extension (CE) movements that are essential for establishing the three germ layers and body axis during early vertebrate development. Here, we describe detailed practical procedures required for the particular studies in Xenopus CE movements

Key words

Convergent extension (CE) movements Planar cell polarity (PCP) Wnt pathway Xenopus development 

Notes

Acknowledgments

We acknowledge Mi-Jung Kim and other members of our laboratory for reading this manuscript and providing constructive criticism. This work was supported by the National Research Foundation of Korea (KRF) grant (No. 20090092829) and K-MeP project (T31130) of Korea Basic Science Institute (KBSI).

References

  1. 1.
    Veeman MT, Axelrod JD, Moon RT. (2003) A second canon. Functions and mechanisms of beta-catenin-independent Wnt signaling. Dev Cell Sep;5(3):367–77.CrossRefGoogle Scholar
  2. 2.
    Wallingford JB, Fraser SE, Harland RM. (2002) Convergent extension: the molecular control of polarized cell movement during embryonic development. Dev Cell Jun;2(6):695–706.CrossRefGoogle Scholar
  3. 3.
    Myers DC, Sepich DS, Solnica-Krezel L. (2002) Convergence and extension in vertebrate gastrulae: cell movements according to or in search of identity? Trends Genet Sep;18(9):447–55.CrossRefGoogle Scholar
  4. 4.
    Wallingford JB, Habas R. (2005) The developmental biology of Dishevelled: an enigmatic protein governing cell fate and cell polarity. Development Oct;132(20):4421–36.CrossRefGoogle Scholar
  5. 5.
    Tada M, Smith JC. (2000) Xwnt11 is a target of Xenopus Brachyury: regulation of gastrulation movements via Dishevelled, but not through the canonical Wnt pathway. Development May;127(10):2227–38.Google Scholar
  6. 6.
    Djiane A, Riou J, Umbhauer M, Boucaut J, Shi D. (2000) Role of frizzled 7 in the regulation of convergent extension movements during gastrulation in Xenopus laevis. Development Jul;127(14):3091–100.Google Scholar
  7. 7.
    Kim GH, Han JK. (2007) Essential role for beta-arrestin 2 in the regulation of Xenopus convergent extension movements. EMBO J May 16;26(10):2513–26.Google Scholar
  8. 8.
    Kim GH, Han JK. (2005) JNK and ROKalpha function in the noncanonical Wnt/RhoA signaling pathway to regulate Xenopus convergent extension movements. Dev Dyn Apr;232(4):958–68.CrossRefGoogle Scholar
  9. 9.
    Wacker S, Grimm K, Joos T, Winklbauer R. (2000) Development and control of tissue separation at gastrulation in Xenopus. Dev Biol Aug 15;224(2):428–39.Google Scholar
  10. 10.
    Winklbauer R, Medina A, Swain RK, Steinbeisser H. (2001) Frizzled-7 signalling controls tissue separation during Xenopus gastrulation. Nature Oct 25;413(6858):856–60.Google Scholar
  11. 11.
    Ren XD, Kiosses WB, Schwartz MA. (1999) Regulation of the small GTP-binding protein Rho by cell adhesion and the cytoskeleton. EMBO J Feb 1;18(3):578–85.Google Scholar
  12. 12.
    Park E, Kim GH, Choi SC, Han JK. (2006) Role of PKA as a negative regulator of PCP signaling pathway during Xenopus gastrulation movements. Dev Biol Apr 15;292(2):344–57.Google Scholar
  13. 13.
    Sive HL, Grainger RM, Harland RM. (2000) Early Development of Xenopus laevis: A Laboratory Manual. Cold Spring Harbor Laboratory Press.Google Scholar
  14. 14.
    Nieuwkoop PD, Faber J. (1967) Normal table of Xenopus laevis (Daudin). Amsterdam: North Holland.Google Scholar

Copyright information

© Springer Science+Business Media, LLC 2012

Authors and Affiliations

  • Gun-Hwa Kim
    • 1
    Email author
  • Edmond Changkyun Park
    • 1
  • Jin-Kwan Han
    • 2
  1. 1.Division of Life ScienceKorea Basic Science InstituteDaejeonRepublic of Korea
  2. 2.Division of Molecular and Life Sciences, Department of Life SciencePohang University of Science and TechnologyPohangRepublic of Korea

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