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Analyzing Frizzled Signaling Using Fixed and Live Imaging of the Asymmetric Cell Division of the Drosophila Sensory Organ Precursor Cell

  • Bertrand Jauffred
  • Yohanns BellaicheEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 839)

Abstract

When you look at the dorsal thorax of a fruitfly, you can easily get fascinated by the high degree of alignment of the bristles that show a strong polarization in their surface organization. This organization of cells in the plane of the epithelium is known as planar cell polarity (PCP), and was initially characterized in Drosophila melanogaster. This process is important in a broad variety of morphological cellular asymmetries in various organisms. In Drosophila, genetic studies of PCP mutants showed that the asymmetric division of the sensory organ precursor cell (pI cell) is polarized along the anterior–posterior axis by Frizzled receptor signaling. Here, we described two methods to image and analyze the PCP in the pI cell model.

Key words

Asymmetric division Sensory organ precursor cell (SOP or pI cell) Notum Dissection Confocal imaging 

Notes

Acknowledgments

The authors would like to thank Rachel Duffie and Boris Guirao for critical reading. Work in Bellaiche lab is supported by grants to Y.B. from the Association pour la Recherche sur le Cancer (ARC 4830), the ANR (BLAN07-3-207540), the CNRS, INSERM, ERC Starting Grant (CePoDro 209718), and the Curie Institute.

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Copyright information

© Springer Science+Business Media, LLC 2012

Authors and Affiliations

  1. 1.Polarity Division and MorphogenesisInstitut Curie, CNRS UMR 3215, INSERM U934ParisFrance

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