The Embryonic Mouse Gut Tube as a Model for Analysis of Epithelial Polarity

  • Makoto Matsuyama
  • Akihiko ShimonoEmail author
Part of the Methods in Molecular Biology book series (MIMB, volume 839)


Recent accumulating data indicate links between planer cell polarity (PCP) and apicobasal (AB) polarity in epithelial cells. PCP regulatory genes have been shown to be involved in the establishment of AB polarity in addition to regulating PCP. We have shown that the gut tube of the mouse embryo is a unique model system for the analysis of epithelial polarities, e.g., oriented cell division and AB polarity, with respect to the PCP pathway. The regulation of epithelial polarity by the PCP pathway might play an essential role in organ morphogenesis relating to physiological function.

Key words

Apicobasal (AB) polarity Oriented cell division Gut tube Confocal microscope Immunofluorescent staining 



This work was supported by the Singapore National Research Foundation and the Ministry of Education under the Research Center of Excellence Program.


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Copyright information

© Springer Science+Business Media, LLC 2012

Authors and Affiliations

  1. 1.Division of BiochemistryAichi Cancer Center Research InstituteNagoyaJapan
  2. 2.Centre of Life Sciences, Cancer Science Institute of SingaporeNational University of SingaporeSingaporeSingapore

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