Proteoglycans pp 323-337 | Cite as

Proteoglycans and Osteolysis

  • Marc Baud’Huin
  • Céline Charrier
  • Gwenola Bougras
  • Régis Brion
  • Frédéric Lezot
  • Marc Padrines
  • Dominique HeymannEmail author
Part of the Methods in Molecular Biology book series (MIMB, volume 836)


Osteolysis is a complex mechanism resulting from an exacerbated activity of osteoclasts associated or not with a dysregulation of osteoblast metabolism leading to bone loss. This bone defect is not compensated by bone apposition or by apposition of bone matrix with poor mechanical quality. Osteolytic process is regulated by mechanical constraints, by polypeptides including cytokines and hormones, and by extracellular matrix components such as proteoglycans (PGs) and glycosaminoglycans (GAGs). Several studies revealed that GAGs may influence osteoclastogenesis, but data are very controversial: some studies showed a repressive effect of GAGs on osteoclastic differentiation, whereas others described a stimulatory effect. The controversy also affects osteoblasts which appear sometimes inhibited by polysaccharides and sometimes stimulated by these compounds. Furthermore, long-term treatment with heparin leads to the development of osteoporosis fueling the controversy. After a brief description of the principal osteoclastogenesis assays, the present chapter summarizes the main data published on the effect of PGs/GAGs on bone cells and their functional incidence on osteolysis.

Key words

Bone resorption Osteoclast Glycosaminoglycan RANKL 


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Copyright information

© Springer Science+Business Media, LLC 2012

Authors and Affiliations

  • Marc Baud’Huin
    • 1
    • 2
  • Céline Charrier
    • 1
    • 2
  • Gwenola Bougras
    • 1
    • 2
  • Régis Brion
    • 1
    • 2
  • Frédéric Lezot
    • 1
    • 2
  • Marc Padrines
    • 3
    • 4
  • Dominique Heymann
    • 3
    • 4
    • 5
    Email author
  1. 1.INSERM, UMR 957NantesFrance
  2. 2.Physiopathologie de la Résorption Osseuse et Thérapie des Tumeurs Osseuses PrimitivesUniversité de Nantes, Nantes Atlantique UniversitésNantesFrance
  3. 3.INSERM, U957NantesFrance
  4. 4.Laboratoire de Physiopathologie de la Résorption Osseuse et Thérapie des Tumeurs Osseuses PrimitivesUniversité de Nantes, Nantes Atlantique UniversitésNantesFrance
  5. 5.Centre Hospitalier Universitaire de NantesNantesFrance

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