Abstract
ATP-dependent nucleosome-remodeling motors use the energy of ATP to alter the accessibility of the underlying DNA. Understanding how these motors alter nucleosome structure can be aided by following changes in histone–DNA contacts in real time. Here, we describe a fluorescence resonance energy transfer-based approach that enables visualization of such changes.
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Shahian, T., Narlikar, G.J. (2012). Analysis of Changes in Nucleosome Conformation Using Fluorescence Resonance Energy Transfer. In: Morse, R. (eds) Chromatin Remodeling. Methods in Molecular Biology, vol 833. Humana Press. https://doi.org/10.1007/978-1-61779-477-3_20
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DOI: https://doi.org/10.1007/978-1-61779-477-3_20
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