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Isolation of NEDDylated Proteins in Human Cells

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Ubiquitin Family Modifiers and the Proteasome

Part of the book series: Methods in Molecular Biology ((MIMB,volume 832))

Abstract

Identification of substrates for post-translational modification with ubiquitin and ubiquitin-like molecules is a key in our understanding of the role of this modification in the regulation of biological processes. The ubiquitin-like molecule NEDD8 plays a vital role in cell growth, viability, and development. In this ­chapter, we describe a method for efficient isolation and detection of NEDDylated proteins in human cells.

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Acknowledgements

Research in DPX laboratory is supported by the Association for International Cancer Research (AICR) and INSERM. D.P.X. is an AICR Research Fellow.

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Authors and Affiliations

  1. Wellcome Trust Centre for Gene Regulation and Expression, College of Life Sciences, University of Dundee, Dundee, Scotland, UK

    Orsolya Leidecker & Dimitris P. Xirodimas

  2. Centre de Recherche de Biochimie Macromoléculaire – UMR 5237, CNRS, Montpellier, France

    Dimitris P. Xirodimas

Authors
  1. Orsolya Leidecker
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  2. Dimitris P. Xirodimas
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Corresponding author

Correspondence to Dimitris P. Xirodimas .

Editor information

Editors and Affiliations

  1. Institut für Genetik, Universität Köln, Zülpicher Str. 47, Köln, 50674, Germany

    R. Jürgen Dohmen

  2. Fak. Biologie, Universität Konstanz, Konstanz, 78457, Germany

    Martin Scheffner

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Leidecker, O., Xirodimas, D.P. (2012). Isolation of NEDDylated Proteins in Human Cells. In: Dohmen, R., Scheffner, M. (eds) Ubiquitin Family Modifiers and the Proteasome. Methods in Molecular Biology, vol 832. Humana Press. https://doi.org/10.1007/978-1-61779-474-2_8

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  • DOI: https://doi.org/10.1007/978-1-61779-474-2_8

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  • Publisher Name: Humana Press

  • Print ISBN: 978-1-61779-473-5

  • Online ISBN: 978-1-61779-474-2

  • eBook Packages: Springer Protocols

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