Abstract
Antibodies that specifically recognize polyubiquitin chains containing ubiquitins linked at a particular lysine residue are powerful tools for interrogating endogenous protein modifications. Here, we describe protocols for revealing K11-, K48-, and K63-linked polyubiquitin chains by western blotting, immunoprecipitation, or immunostaining.
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Newton, K. et al. (2012). Using Linkage-Specific Monoclonal Antibodies to Analyze Cellular Ubiquitylation. In: Dohmen, R., Scheffner, M. (eds) Ubiquitin Family Modifiers and the Proteasome. Methods in Molecular Biology, vol 832. Humana Press. https://doi.org/10.1007/978-1-61779-474-2_13
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DOI: https://doi.org/10.1007/978-1-61779-474-2_13
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Online ISBN: 978-1-61779-474-2
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