Generation and Hepatic Differentiation of Human iPS Cells

  • Tetsuya Ishikawa
  • Keitaro Hagiwara
  • Takahiro Ochiya
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 826)

Abstract

A method for the generation of human induced pluripotent stem (iPS) cells was established. This method employs adenovirus carrying the ecotropic retrovirus receptor mCAT1 and Moloney murine leukemia virus (MMLV)-based retroviral vectors carrying the four transcription factors POU5F1 (OCT3/4), KLF4, SOX2, and MYC (c-Myc) (Masaki H & Ishikawa T Stem Cell Res 1:105–15, 2007). The differentiation of human iPS cells into hepatic cells was performed by a stepwise protocol (Song Z et al. Cell Res 19:1233–42, 2009). These cells have potential as patient-specific in vitro models for studying disease etiology and could be used in drug discovery programs tailored to deal with genetic variations in drug efficacy and toxicity.

Key words

Human iPS cells POU5F1 (OCT3/4) KLF4 Sox2 MYC (c-Myc) Retrovirus Hepatic differentiation 

References

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Copyright information

© Springer Science+Business Media, LLC 2012

Authors and Affiliations

  • Tetsuya Ishikawa
    • 1
  • Keitaro Hagiwara
    • 2
  • Takahiro Ochiya
    • 2
  1. 1.Core Facilities for Research and Innovative MedicineNational cancer Center Research InstituteTokyoJapan
  2. 2.Division of Molecular and Cellular MedicineNational Cancer Center Research InstituteTokyoJapan

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