Efficient Hepatic Differentiation from Human iPS Cells by Gene Transfer

  • Kenji KawabataEmail author
  • Mitsuru Inamura
  • Hiroyuki Mizuguchi
Part of the Methods in Molecular Biology book series (MIMB, volume 826)


Establishment of protocols for the differentiation of hepatic cells from human embryonic stem (ES) and induced pluripotent stem (iPS) cells could contribute to regenerative cell therapies or drug discovery and development. However, the differentiation efficiency of endoderm-derived cells, such as hepatic cells, from human ES and iPS cells is poor because hepatic cells are differentiated via multiple lineages including endodermal cells, hepatic progenitor cells, and mature hepatocytes. We show here the protocols for efficient hepatic differentiation from human ES and iPS cells by adenovirus vector-mediated gene transfer.

Key words

ES cells iPS cells Hepatocytes Adenovirus vector Regenerative medicine Drug development 



This study was supported by grants from the Ministry of Education, Sports, Science and Technology of Japan and by grants from the Ministry of Health, Labor, and Welfare of Japan.


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Copyright information

© Springer Science+Business Media, LLC 2012

Authors and Affiliations

  • Kenji Kawabata
    • 1
    Email author
  • Mitsuru Inamura
    • 2
  • Hiroyuki Mizuguchi
    • 3
  1. 1.Laboratory of Stem Cell RegulationNational Institute of Biomedical InnovationOsakaJapan
  2. 2.Department of Biochemistry and Molecular Biology, Graduate School of Pharmaceutical ScienceOsaka UniversityOsakaJapan
  3. 3.Department of Biochemistry and Molecular Biology, Graduate School of Pharmaceutical SciencesOsaka UniversityOsakaJapan

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