Abstract
For routine genetic analysis of Cannabis sativa, two methods are currently in use, (a) AFLP; amplified fragment length polymorphism analysis and (b) STR; short tandem repeat analysis. The AFLP method used on capillary electrophoresis instrumentation is fully described in this chapter. AFLP analysis generates numerous nonspecific marker fragments for a complex DNA pattern and is available in kit format for quality assurance of reagents. This method is particularly useful when discerning the genetics of highly inbred plant species that may share much of the same DNA with only slight differences due to their common genetic background. AFLP analysis, however, is most effective on fresh or well-preserved plant specimens where the integrity of the DNA is high and the sample is a single source specimen (i.e., not a mixture of plants or different species).
Key words
- Forensic botany
- AFLP
- STR
- Cannabis
- Marijuana
- DNA
- Environmental
- Database
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Acknowledgments
This work was previously supported by the National Institute of Justice Grant #2001-IJ-CX-K011. Thank you to the Connecticut State Forensic Science Laboratory and the University of New Haven for use of their facilities. Numerous students, scientists, and task force personnel offered advice or generated data sets during the course of the grant period. Thank you to Dr. Henry Lee, Major Timothy Palmbach, Dr. Eric Buel, Captain Peter Warren, members of the Connecticut Statewide Narcotics Task Force, Eric Carita, Nicholas C.S. Yang, Elizabeth McClure Baker, Joselle Germano, Edward Jachimowicz III, Shane Lumpkins, Khartika Divarkaran, and Michelle Irvin for their work on the grant funded project.
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Coyle, H.M. (2012). Capillary Electrophoresis of DNA from Cannabis sativa for Correlation of Samples to Geographic Origin. In: Alonso, A. (eds) DNA Electrophoresis Protocols for Forensic Genetics. Methods in Molecular Biology, vol 830. Humana Press. https://doi.org/10.1007/978-1-61779-461-2_17
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DOI: https://doi.org/10.1007/978-1-61779-461-2_17
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