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Capillary Electrophoresis of a Multiplex Reverse Transcription-Polymerase Chain Reaction to Target Messenger RNA Markers for Body Fluid Identification

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DNA Electrophoresis Protocols for Forensic Genetics

Part of the book series: Methods in Molecular Biology ((MIMB,volume 830))

Abstract

The analysis of cell-specific mRNA expression is a promising new method for the identification of body fluids. A number of mRNA markers have been identified for the forensically most relevant body fluids: blood, saliva, semen, vaginal secretions, and menstrual blood. Apart from a significant improvement in specificity compared to conventional protein-based methods, other important advantages of body fluid identification by mRNA profiling include the possibility of simultaneously isolating RNA and DNA from the same piece of stain and the ability to multiplex numerous RNA markers for the identification of one or several body fluids. RNA profiling can be incorporated into current DNA analysis pipelines.

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Author information

Authors and Affiliations

  1. Institute of Legal Medicine, Forensic Genetics, University of Zürich, Zürich, Switzerland

    Cordula Haas

  2. National Center for Forensic Science, University of Central Florida, Orlando, FL, USA

    Erin Hanson & Jack Ballantyne

Authors
  1. Cordula Haas
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  2. Erin Hanson
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  3. Jack Ballantyne
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Corresponding author

Correspondence to Cordula Haas .

Editor information

Editors and Affiliations

  1. Ciencias Forenses, Servicio de Biología, Instituto Nacional de Toxicología y, C/ José Echegaray 4, Las Rozas, 28232, Madrid, Spain

    Antonio Alonso

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Haas, C., Hanson, E., Ballantyne, J. (2012). Capillary Electrophoresis of a Multiplex Reverse Transcription-Polymerase Chain Reaction to Target Messenger RNA Markers for Body Fluid Identification. In: Alonso, A. (eds) DNA Electrophoresis Protocols for Forensic Genetics. Methods in Molecular Biology, vol 830. Humana Press. https://doi.org/10.1007/978-1-61779-461-2_12

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  • DOI: https://doi.org/10.1007/978-1-61779-461-2_12

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  • Publisher Name: Humana Press

  • Print ISBN: 978-1-61779-460-5

  • Online ISBN: 978-1-61779-461-2

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