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Multiple Reaction Monitoring for the Accurate Quantification of Amino Acids: Using Hydroxyproline to Estimate Collagen Content

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Amino Acid Analysis

Part of the book series: Methods in Molecular Biology ((MIMB,volume 828))

Abstract

Multiple reaction monitoring (MRM) mass spectrometry may be regarded as the gold standard methodology for quantitative mass spectrometry and has been adopted for the analysis of small molecules especially within the pharmaceutical industry. It can also be applied to the analysis of peptides and proteins and to the measurement of the basic building blocks of proteins, amino acids. Here, we describe the application of MRM mass spectrometry to the measurement of hydroxyproline after acid hydrolysis of various animal tissues. We show that the measurement of hydroxyproline provides an accurate and reliable estimate of the collagen content of such tissues and may be a useful indicator of meat tenderness.

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Acknowledgements

This work was supported by the Cooperative Research Centre for Sheep Innovation. The authors thank Dr. David Hopkins of NSW Agriculture, Cowra, NSW, Australia and Mr. Malcolm Boyce of Murdoch University, WA, Australia for the supply of the freeze-dried muscle tissue samples used in this study. The authors also thank the Molecular and Cellular Proteomics Facility at The University of Queensland Institute for Molecular Bioscience for access to equipment.

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Correspondence to Michelle L. Colgrave .

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Colgrave, M.L., Allingham, P.G., Tyrrell, K., Jones, A. (2012). Multiple Reaction Monitoring for the Accurate Quantification of Amino Acids: Using Hydroxyproline to Estimate Collagen Content. In: Alterman, M., Hunziker, P. (eds) Amino Acid Analysis. Methods in Molecular Biology, vol 828. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-61779-445-2_23

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  • DOI: https://doi.org/10.1007/978-1-61779-445-2_23

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-61779-444-5

  • Online ISBN: 978-1-61779-445-2

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