Abstract
The Rho family comprises a major branch of the Ras superfamily of small GTPases. A majority of Rho GTPases are synthesized as inactive, cytosolic proteins. They then undergo posttranslational modification by isoprenoid or fatty acid lipids, and together with additional carboxyl-terminal sequences target Rho GTPases to specific membrane and subcellular compartments essential for function. We summarize the use of biochemical and cellular assays and pharmacologic inhibitors instrumental for the study of the role of posttranslational lipid modifications and processing in Rho GTPase biology.
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Acknowledgments
This work was supported, in whole or in part, by National Institutes of Health Grants CA063071, CA67771, and CA92240 to C.J.D.
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Mitin, N., Roberts, P.J., Chenette, E.J., Der, C.J. (2012). Posttranslational Lipid Modification of Rho Family Small GTPases. In: Rivero, F. (eds) Rho GTPases. Methods in Molecular Biology, vol 827. Springer, New York, NY. https://doi.org/10.1007/978-1-61779-442-1_6
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DOI: https://doi.org/10.1007/978-1-61779-442-1_6
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