Assessing Cell Size and Cell Cycle Regulation in Cells with Altered TOR Activity
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Abstract
Target of rapamycin (TOR) regulates the growth of cells and organisms. Numerous growth-promoting and growth-arresting pathways converge on TOR; TOR acts as an important hub, balancing the pro- and anti-growth signals within a cell. Since it regulates growth at the cellular level, cell size can be used as an indirect readout of TOR activity. Here, we describe methods used to analyze cell size in cell culture and in the Drosophila wing.
Key words
mTOR Cell size FACS analysis Coulter counter Cell volume Drosophila siRNAReferences
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