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Stem-Loop RT-qPCR for MicroRNA Expression Profiling

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Next-Generation MicroRNA Expression Profiling Technology

Part of the book series: Methods in Molecular Biology ((MIMB,volume 822))

Abstract

Quantification of the microRNAs (miRNAs) in cells or tissues is a crucial step in understanding their biological functions. Development of the stem-loop reverse transcription procedure and TaqMan® miRNA assays enables accurate detection of miRNA expression levels by quantitative PCR. Increased experimental throughput permits the expression screening of larger number of miRNAs with small amounts of sample. Here, we demonstrate the use of both TaqMan® Array Card and OpenArray® platforms to accurately determine the level of miRNA gene expression in biological samples.

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Acknowledgments

The authors would like to thank members of TaqMan MicroRNA Assays R&D and OpenArray R&D teams at Life Technologies for their support.

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Correspondence to Caifu Chen .

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Hurley, J., Roberts, D., Bond, A., Keys, D., Chen, C. (2012). Stem-Loop RT-qPCR for MicroRNA Expression Profiling. In: Fan, JB. (eds) Next-Generation MicroRNA Expression Profiling Technology. Methods in Molecular Biology, vol 822. Humana Press. https://doi.org/10.1007/978-1-61779-427-8_3

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  • DOI: https://doi.org/10.1007/978-1-61779-427-8_3

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  • Publisher Name: Humana Press

  • Print ISBN: 978-1-61779-426-1

  • Online ISBN: 978-1-61779-427-8

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