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Blood-Based miRNA Preparation for Noninvasive Biomarker Development

Protocol
First Online:
Part of the Methods in Molecular Biology book series (MIMB, volume 822)

Abstract

This chapter describes several methods for the isolation of miRNAs from peripheral whole blood samples or constituent fractions thereof, such as peripheral blood mononuclear cells, plasma, and serum. The methods described here are recently introduced protocols dedicated to the isolation of total RNAs including small RNAs, e.g., miRNeasy Kit and PAXgene Blood miRNA Kit, or alternatively for the enrichment of low-molecular-weight RNA (LMW RNA) fractions including small RNAs, e.g., using the miRNeasy Kit. Furthermore, modifications of classical RNA purification protocols to facilitate the recovery of small RNAs are highlighted.

Key words

miRNA Peripheral blood mononuclear cells Peripheral blood RNA stabilization Low-molecular-weight RNA Small RNA enrichment 
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Notes

Acknowledgments

Protocols referring to the PAXgene Blood miRNA Kit, PAXgene Blood RNA Kit, QIAamp Criculating Nucleic Acid Kit, miRNeasy Mini Kit, RNeasy Mini kit, RNeasy MinElute Cleanup Kit are established by QIAGEN and were mainly cited literally. We are grateful to receive copyright permission.

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Copyright information

© Springer Science+Business Media, LLC 2012

Authors and Affiliations

  1. 1.Life and Medical Sciences (LIMES), Genomics and ImmunoregulationUniversity of BonnBonnGermany
  2. 2.Illumina, Inc.San DiegoUSA
  3. 3.QIAGEN GmbH, Diagnostic Sample Preparation and Stabilization, R&D DepartmentQIAGENHildenGermany

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