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The In Vitro Mammalian Chromosome Aberration Test

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Genetic Toxicology

Part of the book series: Methods in Molecular Biology ((MIMB,volume 817))

Abstract

The short-term in vitro mammalian cell chromosome aberration test is used to assess potential genotoxic hazard of test substances. Mammalian cells are cultured in vitro, exposed to a test substance, harvested, and the frequency of asymmetrical structural chromosome aberrations is measured. Human peripheral blood lymphocytes do not normally divide. The assessment of the effects of cyclophosphamide on lymphocytes, stimulated to divide in whole blood cultures in vitro, is described. Procedures that are important in generating accurate results are emphasised, to avoid false positive results. The study design for a regulatory assay, the use of established cell lines, alternative methods of measuring cytotoxicity and analysis of results are included.

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Acknowledgements

The author would like to thank J. Whitwell and M. Lloyd for their helpful comments, and Covance Laboratories Ltd for providing the photographs.

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Correspondence to Gill Clare .

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Clare, G. (2012). The In Vitro Mammalian Chromosome Aberration Test. In: Parry, J., Parry, E. (eds) Genetic Toxicology. Methods in Molecular Biology, vol 817. Springer, New York, NY. https://doi.org/10.1007/978-1-61779-421-6_5

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  • DOI: https://doi.org/10.1007/978-1-61779-421-6_5

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  • Publisher Name: Springer, New York, NY

  • Print ISBN: 978-1-61779-420-9

  • Online ISBN: 978-1-61779-421-6

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