Quantitation of Amyloid Beta Peptides in CSF by Surface Enhanced MALDI-TOF
Alzheimer’s disease is characterized by the deposition of amyloid plaques in the brain. The major components of these plaques are β-amyloid (Aβ) peptides. The CSF concentration of these peptides can therefore provide a valuable biomarker for potentially predicting the state of disease and/or monitoring the efficacy of a drug aiming to inhibit the formation of amyloid plaques. Although the concentration of a given peptide in CSF can easily be measured by ELISA methods, few methods are able to simultaneously observe and distinguish between various peptides of similar yet slightly different amino acid composition. The Surface Enhanced Laser Desorption/Ionization–Time Of Flight mass spectrometry (SELDI-TOF) technology, a platform combining the use of an antibody and MALDI-TOF, can be used to simultaneously detect and quantitate various Aβ peptides with sensitivities in the picomolar range.
Key wordsβ-Amyloid peptides Aβ40 Aβ42 Alzheimer’s disease MALDI-TOF SELDI-TOF CSF Quantitation
The authors would like to thank Dr. Amanda Bulman for her constructive suggestions and SELDI-related technical support throughout the development of this method.
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