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Isolation, Differentiation, and Characterisation of Skeletal Stem Cells from Human Bone Marrow In Vitro and In Vivo

  • Rahul S. Tare
  • Peter D. Mitchell
  • Janos Kanczler
  • Richard O. C. Oreffo
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 816)

Abstract

In this chapter, we describe techniques for the isolation and characterization of skeletal stem cells from human bone marrow. The methods for enrichment of STRO-1 positive cells using magnetic activated cell sorting are described and we also cover techniques for establishing and characterising osteogenic, adipogenic, and chondrogenic cultures from these cells. Finally, we present methods for studying the ability of these cells to produce bone in vivo using diffusion chambers which have been implanted subcutaneously in mice.

Key words

Stem cells Osteoblast Adipocyte Chondrocyte Bone marrow Diffusion chamber 

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Copyright information

© Springer Science+Business Media, LLC 2012

Authors and Affiliations

  • Rahul S. Tare
    • 1
  • Peter D. Mitchell
    • 1
  • Janos Kanczler
    • 1
  • Richard O. C. Oreffo
    • 1
  1. 1.Bone and Joint Research Group, Centre for Human Development, Stem Cells and Regeneration, Developmental Origins of Health and Disease, Institute of Developmental SciencesUniversity of Southampton Medical SchoolSouthamptonUK

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