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Mechanical Stimulation of Bone Cells Using Fluid Flow

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Book cover Bone Research Protocols

Part of the book series: Methods in Molecular Biology ((MIMB,volume 816))

Abstract

This chapter describes several methods suitable for mechanically stimulating monolayers of bone cells by fluid shear stress (FSS) in vitro. Fluid flow is generated by pumping culture medium through two parallel plates, one of which contains a monolayer of cells. Methods for measuring nitric oxide production by bone cells in response to FSS are also described.

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Authors and Affiliations

  1. Department of Developmental Biology, The Roslin Institute, University of Edinburgh, Edinburgh, UK

    Carmen Huesa

  2. Department of Oral Cell Biology, Academic Centre for Dentistry Amsterdam (ACTA), University of Amsterdam and VU University Amsterdam, Research Institute MOVE, Amsterdam, The Netherlands

    Astrid D. Bakker

Authors
  1. Carmen Huesa
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  2. Astrid D. Bakker
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Corresponding author

Correspondence to Carmen Huesa .

Editor information

Editors and Affiliations

  1. Institute of Medical Sciences, University of Aberdeen, Forester Hill, Aberdeen, AB25 2ZD, United Kingdom

    Miep H. Helfrich

  2. Institute of Genetics and Molecular Medi, Rheumatic Diseases Unit, University of Edinburgh, Crewe Road South, Edinburgh, EH4 2XU, United Kingdom

    Stuart H. Ralston

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Huesa, C., Bakker, A.D. (2012). Mechanical Stimulation of Bone Cells Using Fluid Flow. In: Helfrich, M., Ralston, S. (eds) Bone Research Protocols. Methods in Molecular Biology, vol 816. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-61779-415-5_36

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  • DOI: https://doi.org/10.1007/978-1-61779-415-5_36

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-61779-414-8

  • Online ISBN: 978-1-61779-415-5

  • eBook Packages: Springer Protocols

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