Ovariectomy/Orchidectomy in Rodents

  • Aymen I. IdrisEmail author
Part of the Methods in Molecular Biology book series (MIMB, volume 816)


This chapter describes the surgical procedures for ovariectomy and orchidectomy in mice and rats. In ­addition to providing technical details of the surgical techniques, details of anaesthesia and perioperative care are also included.

Key words

Surgery Ovariectomy Orchidectomy Oestrogen Androgen Rodents Mouse Rat Bone loss 



The author would like to thank Dr. Antonia Sophocleous PhD for providing the images for Figs. 1 and 2 and for critically reviewing the manuscript.


  1. 1.
    Khosla, S., Melton, L. J. III, and Riggs, B. L. (2001) Estrogens and bone health in men. Calcif. Tissue Int. 69, 189–192.PubMedCrossRefGoogle Scholar
  2. 2.
    Khosla, S., Melton, L. J. III, Atkinson, E. J., O’Fallon, W. M., Klee, G. G., and Riggs, B. L. (1998) Relationship of serum sex steroid levels and bone turnover markers with bone mineral density in men and women: a key role for bioavailable estrogen. J. Clin. Endocrinol. Metab. 83, 2266–2274.PubMedCrossRefGoogle Scholar
  3. 3.
    Samuels, A., Perry, M. J., and Tobias, J. H. (1999) High-dose estrogen induces de novo medullary bone formation in female mice. J. Bone Miner. Res. 14, 178–186.PubMedCrossRefGoogle Scholar
  4. 4.
    Tobias, J. H., and Compston, J. E. (1999) Does estrogen stimulate osteoblast function in postmenopausal women? Bone 24, 121–124.PubMedCrossRefGoogle Scholar
  5. 5.
    Weinstein, R. S., and Manolagas, S. C. (2000) Apoptosis and osteoporosis. Am. J. Med. 108, 153–164.PubMedCrossRefGoogle Scholar
  6. 6.
    Raisz, L. G. (2005) Pathogenesis of osteoporosis: concepts, conflicts, and prospects. J. Clin. Invest. 115, 3318–3325.PubMedCrossRefGoogle Scholar
  7. 7.
    Idris, A. I., Van’t Hof, R. J., Greig, I. R., Ridge, S. A., Baker, D., Ross, R. A., and Ralston, S. H. (2005) Regulation of bone mass, bone loss and osteoclast activity by cannabinoid receptors. Nat. Med. 11, 774–779.PubMedCrossRefGoogle Scholar
  8. 8.
    Rissanen, J. P., Suominen, M. I., Peng, Z., Morko, J., Rasi, S., Risteli, J., and Halleen, J. M. (2008) Short-term changes in serum PINP predict long-term changes in trabecular bone in the rat ovariectomy model. Calcif. Tissue Int. 82, 155–161.PubMedCrossRefGoogle Scholar
  9. 9.
    Khosla, S., Melton, L. J. III, and Riggs, B. L. (2001) Estrogens and bone health in men. Calcif. Tissue Int. 69, 189–192.PubMedCrossRefGoogle Scholar
  10. 10.
    Gunness, M., and Orwoll, E. (1995) Early induction of alterations in cancellous and cortical bone histology after orchiectomy in mature rats. J. Bone Miner. Res. 10, 1735–1744.PubMedCrossRefGoogle Scholar
  11. 11.
    Danielsen, C. C., Mosekilde, L., and Andreassen, T. T. (1992) Long-term effect of orchidectomy on cortical bone from rat femur: bone mass and mechanical properties. Calcif. Tissue Int. 50, 169–174.PubMedCrossRefGoogle Scholar
  12. 12.
    Orwoll, E. S. (1996) Androgens as anabolic agents for bone. Trends Endocrinol. Metab. 7, 77–84.PubMedCrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media, LLC 2012

Authors and Affiliations

  1. 1.Bone and Cancer Group, Edinburgh Cancer Research UK Centre and Rheumatic Disease Unit, the Centre of Molecular MedicineUniversity of EdinburghEdinburghUK

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