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Transmission Electron Microscopy of Bone

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Bone Research Protocols

Part of the book series: Methods in Molecular Biology ((MIMB,volume 816))

Abstract

This chapter describes procedures to process mineralized tissues obtained from different sources for transmission electron microscopy (TEM). Methods for fixation, resin embedding, staining of semi-thin sections and ultrathin sections are presented. In addition, attention will be paid to processing of cultured bone explants for TEM analysis.

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References

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Author information

Authors and Affiliations

  1. Department of Oral Cell Biology, Academic Centre for Dentistry Amsterdam (ACTA), University of Amsterdam and VU University Amsterdam, Research Institute MOVE, Amsterdam, The Netherlands

    Vincent Everts

  2. Department of Periodontology, Academic Centre for Dentistry Amsterdam (ACTA), University of Amsterdam and VU University Amsterdam, Amsterdam, The Netherlands

    Anneke Niehof

  3. Department of Cell Biology and Histology, Academic Medical Centre (AMC), University of Amsterdam, Amsterdam, The Netherlands

    Wikky Tigchelaar-Gutter

  4. Department of Oral Cell Biology and Dept. Periodontology Academic Centre for Dentistry Amsterdam (ACTA), University of Amsterdam and VU University Amsterdam, Research Institute MOVE, Amsterdam, The Netherlands

    Wouter Beertsen

Authors
  1. Vincent Everts
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  2. Anneke Niehof
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  3. Wikky Tigchelaar-Gutter
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  4. Wouter Beertsen
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Corresponding author

Correspondence to Vincent Everts .

Editor information

Editors and Affiliations

  1. Institute of Medical Sciences, University of Aberdeen, Forester Hill, Aberdeen, AB25 2ZD, United Kingdom

    Miep H. Helfrich

  2. Institute of Genetics and Molecular Medi, Rheumatic Diseases Unit, University of Edinburgh, Crewe Road South, Edinburgh, EH4 2XU, United Kingdom

    Stuart H. Ralston

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© 2012 Springer Science+Business Media, LLC

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Everts, V., Niehof, A., Tigchelaar-Gutter, W., Beertsen, W. (2012). Transmission Electron Microscopy of Bone. In: Helfrich, M., Ralston, S. (eds) Bone Research Protocols. Methods in Molecular Biology, vol 816. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-61779-415-5_23

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  • DOI: https://doi.org/10.1007/978-1-61779-415-5_23

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-61779-414-8

  • Online ISBN: 978-1-61779-415-5

  • eBook Packages: Springer Protocols

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