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Extraction of Nucleic Acids from Bone

  • Alun Hughes
  • Tracy L. Stewart
  • Val Mann
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 816)

Abstract

Here, we present methods for extracting DNA and RNA from samples of whole bone tissue and culture bone cells and describe methods quantitative and qualitative measurement of the extracted nucleic acids. These protocols described provide high-quality nucleic acids suitable for downstream applications such as quantitative PCR and microarrays.

Key words

RNA extraction DNA extraction RNA quantification DNA quantification RNA integrity 

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Copyright information

© Springer Science+Business Media, LLC 2012

Authors and Affiliations

  1. 1.Musculoskeletal Research Programme, Division of Applied Medicine, Institute of Medical SciencesUniversity of AberdeenAberdeenUK
  2. 2.School of Health, Science and Social CareUniversity Campus SuffolkIpswichUK

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