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Analysis of Transcriptional Regulation in Bone Cells

  • Huilin Jin
  • Stuart H. Ralston
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 816)

Abstract

Transcription is the process by which the rate of RNA synthesis is regulated. Here, we describe the techniques for carrying out promoter-reporter assays, electrophoretic mobility shift assays, and chromatin immunoprecipitation assays, three commonly used methods for studying gene transcription.

Key words

Transcription Chromatin Immunoprecipitation ChIP EMSA Promoter Gene 

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Copyright information

© Springer Science+Business Media, LLC 2012

Authors and Affiliations

  1. 1.Kennedy Institute of RheumatologyLondonUK
  2. 2.Rheumatic Diseases Unit, Western General HospitalUniversity of EdinburghEdinburghUK

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