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Transfection of Osteoclasts and Osteoclast Precursors

  • Julie C. Crockett
  • David J. Mellis
  • Adam Taylor
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 816)

Abstract

Osteoclasts and their precursors have traditionally been considered difficult cells to transfect using standard approaches. Here, we describe several methods for transfection of mature osteoclasts and their precursors using the Amaxa™ Nucleofector system, lentiviruses, and adenoviruses.

Key words

Osteoclast Transfection Adenovirus Lentivirus 

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Copyright information

© Springer Science+Business Media, LLC 2012

Authors and Affiliations

  • Julie C. Crockett
    • 1
  • David J. Mellis
    • 2
  • Adam Taylor
    • 1
  1. 1.Musculoskeletal Research Programme, Division of Applied Medicine, Institute of Medical SciencesUniversity of AberdeenAberdeenUK
  2. 2.Musculoskeletal Research Programme, Division of Applied Medicine, Institute of Medical SciencesUniversity of AberdeenAberdeenUK

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