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Purifi cation of Multiprotein Histone Acetyltransferase Complexes

  • Yuan-Liang Wang
  • Francesco Faiola
  • Ernest MartinezEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 809)

Abstract

The reversible acetylation of specific lysine residues on core histones regulates gene transcription in eukaryotes. Since the discovery of GCN5 as the first transcription-regulating histone acetyltransferase (HAT), a variety of HATs have now been identified and shown to acetylate different sites on histones as well as on non-histone proteins, including transcription regulators. In general, purified recombinant HATs expressed in bacteria or in insect cells are able to acetylate free histones and sometimes other substrates in vitro. However, such activity is often restricted to certain substrates and/or is very weak on physiological substrates, such as nucleosomes. Moreover, it does not reflect the actual scenario inside the cell, where HATs generally associate with other proteins to form stable multisubunit complexes. Importantly, these peripheral proteins significantly influence the functions of the catalytic HAT subunit by regulating its intrinsic catalytic activity and/or by modulating its target substrate selectivity. In this chapter, we describe detailed methods for the rapid (two step) and efficient purification of large, multiprotein HAT complexes from nuclear extracts of mammalian epitope-tagged cell lines, including protocols for the generation and large-scale suspension culture of these cell lines. These methods have been used to purify and characterize different human GCN5 HAT complexes that retain activity toward their physiological substrates in vitro.

Key words

Transcription Histone acetyltransferase Protein complexes GCN5 Affinity purification FLAG tag S-Sepharose Cell line 

Notes

Acknowledgments

The authors would like to thank Dr. Jennifer Liu for advice. This work was supported by grants R01CA100464 and MCB0448488 from NIH and NSF, respectively.

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Copyright information

© Springer Science+Business Media, LLC 2012

Authors and Affiliations

  • Yuan-Liang Wang
    • 1
  • Francesco Faiola
    • 1
  • Ernest Martinez
    • 1
    Email author
  1. 1.Department of BiochemistryUniversity of CaliforniaRiversideUSA

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