Approaches for Studying Nucleosome Movement by ATP-Dependent Chromatin Remodeling Complexes

  • Swetansu K. Hota
  • Blaine BartholomewEmail author
Part of the Methods in Molecular Biology book series (MIMB, volume 809)


Packaging DNA into compact chromatin enables eukaryotic cells to organize and regulate their genome. Packaging is achieved by wrapping ∼146–147 bp of DNA around a histone octamer to form a nucleosome, the basic unit of chromatin. Chromatin is a barrier of the bound DNA to factors involved in DNA-dependent processes such as transcription, replication, recombination, and repair. Several multisubunit protein complexes can move nucleosome to different positions on DNA utilizing energy derived from ATP hydrolysis and thereby facilitate access to DNA. Several methods are described for measuring nucleosome movement both in vivo and in vitro which provide important insights into the remodeling process.

Key words

Nucleosome position Indirect end-labeling Chromatin remodeling Histone-DNA cross-linking SWI/SNF ISW2 


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Copyright information

© Springer Science+Business Media, LLC 2012

Authors and Affiliations

  1. 1.Southern Illinois University School of MedicineCarbondaleUSA

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