Production and Purification of Recombinant Adeno-Associated Vectors

  • Lijun Wang
  • Véronique Blouin
  • Nicole Brument
  • Mahajoub Bello-Roufai
  • Achille FrancoisEmail author
Part of the Methods in Molecular Biology book series (MIMB, volume 807)


The use of recombinant adeno-associated virus (rAAV) vectors in gene therapy for preclinical studies in animal models and human clinical trials is increasing, as these vectors have been shown to be safe and to mediate persistent transgene expression in vivo. Constant improvement in rAAV manufacturing processes (upstream production and downstream purification) has paralleled this evolution to meet the needs for larger vector batches, higher vector titer, and improved vector quality and safety. This chapter provides an overview of existing production and purification systems used for adeno-associated virus (AAV) vectors, and the advantages and disadvantages of each system are outlined. Regulatory guidelines that apply to the use of these systems for clinical trials are also presented. The methods described are examples of protocols that have been utilized for establishing rAAV packaging cell lines, production of rAAV vectors using recombinant HSV infection, and for chromatographic purification of various AAV vector serotypes. A protocol for the production of clinical-grade rAAV type 2 vectors using transient transfection and centrifugation-based purification is also described.

Key words

Adeno-associated virus rAAV Vector Production Purification Gene therapy Clinical trial cGMP 


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Copyright information

© Springer Science+Business Media, LLC 2012

Authors and Affiliations

  • Lijun Wang
    • 1
  • Véronique Blouin
    • 2
  • Nicole Brument
    • 3
  • Mahajoub Bello-Roufai
    • 1
  • Achille Francois
    • 2
    Email author
  1. 1.Center of Excellence for Regenerative Health BiotechnologyUniversity of FloridaAlachuaUSA
  2. 2.Laboratoire de Thérapie Génique, INSERMUniversité de NantesNantesFrance
  3. 3.INSERM UMR649, Institut de Recherche Thérapeutique - IRT1Université de NantesNantes, Cedex 01France

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