Human Hepatocytes: Isolation, Culture, and Quality Procedures

  • Daniel Knobeloch
  • Sabrina Ehnert
  • Lilianna Schyschka
  • Peter Büchler
  • Michael Schoenberg
  • Jörg Kleeff
  • Wolfgang E. Thasler
  • Natascha C. Nussler
  • Patricio Godoy
  • Jan Hengstler
  • Andreas K. Nussler
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 806)

Abstract

The use of isolated human liver cells in research and development has gained increasing interest during the past years. The possible application may vary between elucidation of new biochemical pathways in liver diseases, drug development, safety issues, and new therapeutic strategies up to direct clinical translation for liver support. However, the isolation of human liver cells requires a well-developed logistic network among surgeons, biologists, and technicians to obtain a high quality of cells. Our laboratories have been involved in various applications of human liver cells and we have long-lasting experiences in human liver cell isolation and their application in R&D. We here summarize the present protocol of our laboratories for cell isolation from normal resected liver tissue, the most common tissue available. In addition, we discuss the necessary network in the clinic and quality controls to maintain human liver cells in culture and the effect of 3D extracellular matrix in cultured cells which results in preservation of hepatocyte epithelial polarity in the form of bile canaliculi and repression of epithelial to mesenchymal transitions occurring in 2D cultures.

Key words

Human hepatocytes Cell isolation Hepatocyte culture Quality control Metabolic competence 

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Copyright information

© Springer Science+Business Media, LLC 2012

Authors and Affiliations

  • Daniel Knobeloch
    • 1
  • Sabrina Ehnert
    • 2
  • Lilianna Schyschka
    • 3
  • Peter Büchler
    • 4
  • Michael Schoenberg
    • 5
  • Jörg Kleeff
    • 4
  • Wolfgang E. Thasler
    • 6
  • Natascha C. Nussler
    • 7
  • Patricio Godoy
    • 8
  • Jan Hengstler
    • 8
  • Andreas K. Nussler
    • 2
  1. 1.Cytonet GmbhWeinheimGermany
  2. 2.Department of TraumatologyEberherd karls Universität TübingenTübingenGermany
  3. 3.Department of TraumatologyMRI, Technische Universität MünchenMunichGermany
  4. 4.Department of General SurgeryTU Munich, MRIMunichGermany
  5. 5.Department of Gastroenterology & HepatologyMedical University HospitalTuebingenGermany
  6. 6.Department of SurgeryLudwigs-Maximillian Universität MünchernMunichGermany
  7. 7.Department of General and Visceral SurgeryKlinikum NeuperlachMunichGermany
  8. 8.IFADO, Leibniz Research Centre for Working Environment and Human FactorsDortmund UniversityDortmundGermany

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