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Electrochemical Aptamer Sensor for Small Molecule Assays

  • Xin Liu
  • Wang Li
  • Xiahong Xu
  • Jiang Zhou
  • Zhou NieEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 800)

Abstract

Detection and quantification of small molecules have played essential roles in environmental analysis and clinical diagnosis. Aptamers are oligonucleic acids that bind to a specific target molecule with high specificity and affinity which are promising features for sensing small molecules. Electrochemical detection is an attractive way to exploit aptamer sensors (aptasensors) because of its high sensitivity, simple instrumentation, low cost, fast response and portability. Herein, we describe a label-free small molecular aptasensor based on a signal-amplification mechanism which uses gold nanoparticles. This aptasensor can selectively detect low nanomolar levels of ATP, the example target compound.

Key words

Aptamer Electrochemical sensor Gold nanoparticles Aptasensor Signal-amplification Chronocoulometry ATP 

Notes

Acknowledgments

This work was funded by the National Natural Science Foundation of China (Nos. 20805013, 20907013), the National Basic Research Program of China (973 Program, Nos. 2009CB421601, 2011CB911002), the Natural Science Foundation of Hunan Province (Nos. 09JJ4006, 10JJ2005), and SKLCBSC-2010-01. We acknowledge Elsevier for the original publication of Fig. 4 in Talanta.

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Copyright information

© Springer Science+Business Media, LLC 2012

Authors and Affiliations

  • Xin Liu
    • 1
  • Wang Li
    • 1
  • Xiahong Xu
    • 1
  • Jiang Zhou
    • 1
  • Zhou Nie
    • 1
    Email author
  1. 1.State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical EngineeringHunan UniversityChangshaChina

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