Chemical Genomics and Proteomics

Volume 800 of the series Methods in Molecular Biology pp 85-94


Profiling Cellular Myristoylation and Palmitoylation Using ω-Alkynyl Fatty Acids

  • Rami N. HannoushAffiliated withDepartment of Early Discovery Biochemistry, Genentech, Inc. Email author 

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Methods to detect and characterize cellular protein myristoylation and palmitoylation are invaluable in cell biology, immunology, and virology. Recently, we developed ω-alkynyl fatty acid probes for monitoring myristoylation and palmitoylation of cellular proteins. This article describes a biochemical procedure for metabolic labeling of cells with ω-alkynyl fatty acids and click chemistry.

Key words

Myristoylation Palmitoylation Fatty acylation Lipids Alkynyl fatty acids Chemical probes Metabolic labeling Click chemistry