Profiling Cellular Myristoylation and Palmitoylation Using ω-Alkynyl Fatty Acids

Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 800)

Abstract

Methods to detect and characterize cellular protein myristoylation and palmitoylation are invaluable in cell biology, immunology, and virology. Recently, we developed ω-alkynyl fatty acid probes for monitoring myristoylation and palmitoylation of cellular proteins. This article describes a biochemical procedure for metabolic labeling of cells with ω-alkynyl fatty acids and click chemistry.

Key words

Myristoylation Palmitoylation Fatty acylation Lipids Alkynyl fatty acids Chemical probes Metabolic labeling Click chemistry 

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Copyright information

© Springer Science+Business Media, LLC 2012

Authors and Affiliations

  1. 1.Department of Early Discovery BiochemistryGenentech, Inc.South San FranciscoUSA

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