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Preparation of Photo-Cross-Linked Small Molecule Affinity Matrices for Affinity Selection of Protein Targets for Biologically Active Small Molecules

  • Hiroshi Takayama
  • Takashi Moriya
  • Naoki KanohEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 800)

Abstract

Small molecule-immobilized affinity matrices are indispensable tools in chemical genomics to screen and purify protein targets for biologically active small molecules. Usually, prior to immobilization, small ­molecules have to be derivatized at a position that does not significantly abrogate the intrinsic biological activity, or chemically synthesized to have an appropriate functional group for the immobilization chemistry. Here, we describe a photo-cross-linking technique to immobilize biologically active small molecules for protein target screening, without the need for chemical derivatization.

Key words

Photo-cross-linking Affinity matrices Biologically active small molecule Immobilization Diazirine Photo-generated carbine 

Notes

Acknowledgments

This work is supported by a Grant-in-Aid for Young Scientists (A) (No. 19681023; to NK), a Grant-in-Aid for Scientific Research (B) (No. 22310132; to NK), and a Grant-in-Aid for JAPS fellows (No. 2255053; to HT). We also thank Prof. Takayuki Doi and Dr. Masahito Yoshida for their assistance with the LC/MS analysis.

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Copyright information

© Springer Science+Business Media, LLC 2012

Authors and Affiliations

  1. 1.Graduate School of Pharmaceutical SciencesTohoku UniversitySendaiJapan

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