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Glycan Microarrays

  • Xuezheng Song
  • Jamie Heimburg-Molinaro
  • David F. Smith
  • Richard D. CummingsEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 800)

Abstract

Glycan microarrays are presentations of multiple glycans or glycoconjugates printed on a single slide for screening with glycan-binding proteins (GBPs), which include lectins, antibodies, bacteria, and viruses. Glycans derivatized with functional groups can be immobilized onto appropriately activated glass slides to generate glycan microarrays where each glycan is printed at similar concentrations. Here we describe a method for fluorescently and functionally derivatizing free reducing glycans, printing microarrays, and interrogating the microarrays with GBPs.

Key words

Glycan Microarray Lectins Glycan-binding proteins Fluorescent labeling 

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Copyright information

© Springer Science+Business Media, LLC 2012

Authors and Affiliations

  • Xuezheng Song
    • 1
  • Jamie Heimburg-Molinaro
    • 1
  • David F. Smith
    • 1
  • Richard D. Cummings
    • 1
    Email author
  1. 1.Department of Biochemistry and The Glycomics CenterEmory University School of MedicineAtlantaUSA

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