Abstract
Neisseria meningitidis is a human specific organism that causes severe sepsis and/or meningitis with high mortality. The disease scenario is rapid and much remains unknown about the disease process and host–pathogen interaction. In this chapter, we describe a protocol for generating a bioluminescently labeled N. meningitidis strain in order to advance our understanding of meningococcal disease progression. We also describe how in vivo bioluminescence imaging (BLI) can be used to observe novel features of the disease dynamics during meningococcal infection.
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Acknowledgments
We thank Caliper Life Sciences for kindly providing the plasmid pXen-13, which was used to construct the plasmids described in this protocol. This work was funded by The Swedish Research Council, Swedish Cancer Society, Magnus Bergvalls Foundation, Ake Wibergs Foundation, Swedish Society for Medicine, Torsten och Ragnar Söderderbergs Foundation, Knut och Alice Wallenbergs Foundation, Laerdal Foundation for Acute Medicine, and Uppsala University.
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Sjölinder, H., Jonsson, AB. (2012). In Vivo Imaging of Meningococcal Disease Dynamics. In: Christodoulides, M. (eds) Neisseria meningitidis. Methods in Molecular Biology, vol 799. Humana, Totowa, NJ. https://doi.org/10.1007/978-1-61779-346-2_10
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DOI: https://doi.org/10.1007/978-1-61779-346-2_10
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Publisher Name: Humana, Totowa, NJ
Print ISBN: 978-1-61779-345-5
Online ISBN: 978-1-61779-346-2
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