Abstract
Optical imaging of luciferage gene expression has become a powerful tool to track cells and viruses in vivo in small animal models. Luciferase imaging has been used to study the location of infection by replication-defective and replication-competent viruses and to track changes in the distribution of viruses in mouse models. This approach has also been used in oncolytic studies as a noninvasive means to monitor the growth and killing of tumor cells modified with luciferase genes. In this chapter, we describe the techniques used for luciferase imaging as have been applied to track replication-defective and replication-competent adenoviruses in mouse and hamster models of oncolysis and virus pharmacology. Although these methods are simple, the process of obtaining accurate luciferase imaging data has many caveats that are discussed.
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References
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Acknowledgments
We would like to thank all past and present Barry laboratory members for their experiences in imaging that have been distilled here. This work has been supported by the Muscular Dystrophy Association, the Ralph C. Wilson Foundation, the Propionic Acidemia Foundation, R01 AI06709, R01 AI065304, R01 CA136945-01A2, and by the NIH P50 CA91956 Prostate Cancer SPORE grant at the Mayo Clinic.
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Barry, M.A., May, S., Weaver, E.A. (2012). Imaging Luciferase-Expressing Viruses. In: Kirn, D., Liu, TC., Thorne, S. (eds) Oncolytic Viruses. Methods in Molecular Biology, vol 797. Humana, Totowa, NJ. https://doi.org/10.1007/978-1-61779-340-0_6
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DOI: https://doi.org/10.1007/978-1-61779-340-0_6
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