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Oncolytic Herpes Simplex Virus Engineering and Preparation

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Oncolytic Viruses

Part of the book series: Methods in Molecular Biology ((MIMB,volume 797))

Abstract

Herpes simplex virus-1 (HSV-1) is an enveloped, double-stranded DNA virus that has been used with modification as an oncolytic virus against a number of tumor types. Modifications that make HSV-1 replication-­conditional, i.e., selectively divide in replicating cells make it fulfill a prerequisite criteria for oncolytic viruses. Other appealing features of HSV-1 as an oncolytic virus include its large, modifiable genome; its sensitivity to antiviral agents, such as ganciclovir; and its lack of host cell integration. Here, we review the methods of HSV-1 engineering, through traditional recombination techniques as well as through bacterial artificial chromosome (BAC) technology. We then describe protocols for titering, amplification, and purification of engineered HSV-1-derived oncolytic viruses.

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Acknowledgments

The authors wish to thank Drs. Samuel Rabkin, Riyuchi Kanai, and Slawomir Antoszczyk for the figures and assistance. PKA is supported by a continuing clinical research grant from the Doris Duke Charitable Foundation. MKA was supported by a research grant from the American Brain Tumor Association (ABTA) at the time studies cited in this chapter were completed.

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Authors and Affiliations

  1. Harvard Medical School, Boston, MA, USA

    Pankaj K. Agarwalla

  2. Department of Neurosurgery, University of California at San Francisco, San Francisco, CA, USA

    Manish K. Aghi

Authors
  1. Pankaj K. Agarwalla
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  2. Manish K. Aghi
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Corresponding author

Correspondence to Manish K. Aghi .

Editor information

Editors and Affiliations

  1. Jennerex Biotherapeutics, Inc., Market Street 1, San Francisco, 94105, California, USA

    David H. Kirn

  2. School of Medicine, Dept. Pathology & Immunology, Washington University, S. Euclid Ave. 660, St.Louis, 63110, Missouri, USA

    Ta-Chiang Liu

  3. Hillman Cancer Center, University of Pittsburgh, Centre Avenue 5117, Pittsburgh, 15213, Pennsylvania, USA

    Stephen H. Thorne

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Agarwalla, P.K., Aghi, M.K. (2012). Oncolytic Herpes Simplex Virus Engineering and Preparation. In: Kirn, D., Liu, TC., Thorne, S. (eds) Oncolytic Viruses. Methods in Molecular Biology, vol 797. Humana, Totowa, NJ. https://doi.org/10.1007/978-1-61779-340-0_1

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  • DOI: https://doi.org/10.1007/978-1-61779-340-0_1

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  • Publisher Name: Humana, Totowa, NJ

  • Print ISBN: 978-1-61779-339-4

  • Online ISBN: 978-1-61779-340-0

  • eBook Packages: Springer Protocols

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