Abstract
Chemoselective reactions are important tools for the modification of peptides and proteins. Thereby the modification is desired to be site specific and bioorthogonal. Here we describe the site-specific modification of azido-proteins via a Staudinger-type phosphite ligation. The reaction was carried out in aqueous system on proteins containing p-azido-phenylalanine in a single position introduced by the amber codon technique. A selective introduction of branched polyethylene scaffolds can be achieved with the application of the methodology reported herein.
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Acknowledgments
The authors acknowledge financial support from the German Science Foundation (DFG) within the Emmy-Noether program (HA 4468/2-1), the SFB 765, the Fonds der Chemischen Industrie (FCI) and the Böhringer-Ingelheim Foundation (“Plus 3-Perspektiven Programm”).
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Majkut, P., Böhrsch, V., Serwa, R., Gerrits, M., Hackenberger, C.P.R. (2012). Site-Specific Modification of Proteins by the Staudinger-Phosphite Reaction. In: Pollegioni, L., Servi, S. (eds) Unnatural Amino Acids. Methods in Molecular Biology, vol 794. Humana Press. https://doi.org/10.1007/978-1-61779-331-8_15
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DOI: https://doi.org/10.1007/978-1-61779-331-8_15
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