N-Linked Global Glycan Profiling by NanoLC Mass Spectrometry

Part of the Methods in Molecular Biology book series (MIMB, volume 790)


A method is detailed for the global profiling of underivatized N-linked glycans that are derived from complex protein mixtures. The method consists of five main steps that include the following: (1) protein denaturation; (2) enzymatic digestion; (3) solid phase extraction; (4) nanoLC MS analysis; and (5) data interpretation. Materials, methods, and algorithms for the identification of both glycan composition and structure are summarized. In addition, potential problems and their resolutions are addressed.

Key words

N-linked glycans NanoLC mass spectrometry Glycoproteins Glycosylation Glycan profiling Hydrophilic interaction chromatography Glycobiology Glycan algorithms 


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Copyright information

© Springer Science+Business Media, LLC 2011

Authors and Affiliations

  1. 1.Department of Genome SciencesUniversity of WashingtonSeattleUSA
  2. 2.Department of ChemistryNorth Carolina State UniversityRaleighUSA

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