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N-Linked Global Glycan Profiling by NanoLC Mass Spectrometry

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Nanoproteomics

Part of the book series: Methods in Molecular Biology ((MIMB,volume 790))

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Abstract

A method is detailed for the global profiling of underivatized N-linked glycans that are derived from complex protein mixtures. The method consists of five main steps that include the following: (1) protein denaturation; (2) enzymatic digestion; (3) solid phase extraction; (4) nanoLC MS analysis; and (5) data interpretation. Materials, methods, and algorithms for the identification of both glycan composition and structure are summarized. In addition, potential problems and their resolutions are addressed.

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Author information

Authors and Affiliations

  1. Department of Genome Sciences, University of Washington, Seattle, WA, USA

    Michael S. Bereman

  2. Department of Chemistry, North Carolina State University, Raleigh, NC, USA

    David C. Muddiman

Authors
  1. Michael S. Bereman
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  2. David C. Muddiman
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Corresponding author

Correspondence to David C. Muddiman .

Editor information

Editors and Affiliations

  1. , Department of Neurosurgery, Geisinger Health Systems, North Academy Avenue 100, Danville, 17822, Pennsylvania, USA

    Steven A. Toms

  2. Neurological Institute, Brain Tumor & Neuro-Oncology Center, The Cleveland Clinic, Euclid Avenue 9500, Cleveland, 44195, Ohio, USA

    Robert J. Weil

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Bereman, M.S., Muddiman, D.C. (2011). N-Linked Global Glycan Profiling by NanoLC Mass Spectrometry. In: Toms, S., Weil, R. (eds) Nanoproteomics. Methods in Molecular Biology, vol 790. Humana Press. https://doi.org/10.1007/978-1-61779-319-6_7

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  • DOI: https://doi.org/10.1007/978-1-61779-319-6_7

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  • Publisher Name: Humana Press

  • Print ISBN: 978-1-61779-318-9

  • Online ISBN: 978-1-61779-319-6

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