Abstract
Noncoding regulatory genomic elements are central for cellular function, differentiation, and disease, but remain poorly characterized. FAIRE (formaldehyde-assisted isolation of regulatory elements) has emerged as a simple method to identify and analyze active regulatory sequences based on their decreased nucleosomal content. More recently FAIRE was combined with high-throughput sequencing (FAIRE-seq) to locate tissue-specific regulatory elements at a genome scale in purified human pancreatic islets. Here we describe the implementation of the FAIRE method in human pancreatic islet cells.
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Acknowledgments
The authors would like to thank Jason D. Lieb and Paul G. Giresi for advice and support in the adaptation of the FAIRE protocol for human islets. Work in the authors’ laboratory has been funded by Ministerio de Ciencia e Innovación and Centro de Investigación Biomédica en Red (CIBER) de Diabetes y Enfermedades Metabólicas.
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Nammo, T., Rodríguez-Seguí, S.A., Ferrer, J. (2011). Mapping Open Chromatin with Formaldehyde-Assisted Isolation of Regulatory Elements. In: Tollefsbol, T. (eds) Epigenetics Protocols. Methods in Molecular Biology, vol 791. Humana Press. https://doi.org/10.1007/978-1-61779-316-5_21
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DOI: https://doi.org/10.1007/978-1-61779-316-5_21
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