Skip to main content

Advances in Epigenetic Technology

  • Protocol
  • First Online:
Epigenetics Protocols

Part of the book series: Methods in Molecular Biology ((MIMB,volume 791))

Abstract

Epigenetics refers to the collective heritable changes in phenotype that arise independent of genotype. Two broad areas of epigenetics are DNA methylation and histone modifications and numerous techniques have been invented to analyze epigenetic processes not only at the level of specific genes, but also to analyze epigenetic changes that occur in defined regions of the genome as well as genome-wide. Advances have also been made in techniques devised to assess the enzymes that mediate epigenetic processes. These methods that are currently driving the field of epigenetics will greatly facilitate continued expansion of this exponentially growing discipline of genetics.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Protocol
USD 49.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 89.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 119.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book
USD 169.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

References

  1. Robertson, K.D. (2001) DNA methylation, methyltransferases and cancer. Oncogene. 20, 3139–3155.

    Article  PubMed  CAS  Google Scholar 

  2. Okano, M., Bell, D.W., Haber, D.A., and Li, E. (1999) Dnmt3a and Dnmt3b are essential for de novo methylation and mammalian development. Cell. 99, 247–257.

    Article  PubMed  CAS  Google Scholar 

  3. Lai, S.R., Phipps, S.M., Liu, L., Andrews, L.G., and Tollefsbol, T.O. (2005) Epigenetic control of telomerase and modes of telomere maintenance in aging and abnormal systems. Front Biosci. 10, 1779–1796.

    Article  PubMed  CAS  Google Scholar 

  4. Lehrmann, H., Pritchard, L.L., and Harel-Bellan, A. (2002) Histone acetyltransferases and deacetylases in the control of cell proliferation and differentiation. Adv Cancer Res. 86, 41–65.

    Article  PubMed  CAS  Google Scholar 

  5. Fuks, F., Burgers, W. A., Brehm, A., Hughes-Davies, L., and Kouzarides, T. (2000) DNA methyltransferase Dnmt1 associates with histone deacetylase activity. Nat Genet. 24, 88–91.

    Article  PubMed  CAS  Google Scholar 

  6. Rauch, T., and Pfeifer, G. (2005) Methylated-CpG island recovery assay: a new technique for the rapid detection of methylated-CpG islands in cancer. Lab Invest. 85, 1172–1180.

    Article  PubMed  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to Trygve O. Tollefsbol .

Editor information

Editors and Affiliations

Rights and permissions

Reprints and permissions

Copyright information

© 2011 Springer Science+Business Media, LLC

About this protocol

Cite this protocol

Tollefsbol, T.O. (2011). Advances in Epigenetic Technology. In: Tollefsbol, T. (eds) Epigenetics Protocols. Methods in Molecular Biology, vol 791. Humana Press. https://doi.org/10.1007/978-1-61779-316-5_1

Download citation

  • DOI: https://doi.org/10.1007/978-1-61779-316-5_1

  • Published:

  • Publisher Name: Humana Press

  • Print ISBN: 978-1-61779-315-8

  • Online ISBN: 978-1-61779-316-5

  • eBook Packages: Springer Protocols

Publish with us

Policies and ethics