Separation-Induced Depression in the Mouse

  • Richard E. Brown
  • Alison L. Martin
  • Rhian K. Gunn
Part of the Neuromethods book series (NM, volume 63)


This chapter outlines the experimental methods for inducing Depression-like behavior in mice by separating group-housed mice to individual housing. This is a model of loneliness in mice. Loneliness-induced depression is measured using the forced swim test (FST) and tail suspension test (TST). The first part of the chapter describes the methods for inducing separation-induced depression. It summarizes the experimental protocols including behavioral testing of the animals. The second part describes some of the issues to be concerned within this paradigm. An up-to-date collection of protocols and procedures for troubleshooting are provided.

Key words

Depression Social stress Mouse model Anxiety FST TST Behavior Social isolation 



This research was funded by NSERC of Canada.


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Copyright information

© Springer Science+Business Media, LLC 2011

Authors and Affiliations

  • Richard E. Brown
    • 1
  • Alison L. Martin
    • 1
  • Rhian K. Gunn
    • 1
  1. 1.Psychology Department and Neuroscience InstituteDalhousie UniversityHalifaxCanada

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