Abstract
This protocol describes an ELISA-based method for assaying serum levels of autoantibodies reactive with neuropeptides. The method allows for measuring relative amounts of free and bound, i.e., those present in immune complexes, autoantibodies using two types of sample buffers providing normal and dissociative conditions, respectively. This method can be applied to measure autoantibody levels directed against other than neuropeptide molecules and in a variety of biological fluids.
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Acknowledgements
The author is thankful to Dr. Danièle Gilbert, Inserm U519, Rouen University, France and to late Dr. Ann Kari Lefvert, Karolinska Institutet, Stockholm, Sweden, for help with protocol development, as well as to the members of the ADEN Laboratory, Rouen University, France whose contribution was crucial to repeatedly reproduce this protocol.
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Fetissov, S.O. (2011). Neuropeptide Autoantibodies Assay. In: Merighi, A. (eds) Neuropeptides. Methods in Molecular Biology, vol 789. Humana Press. https://doi.org/10.1007/978-1-61779-310-3_19
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DOI: https://doi.org/10.1007/978-1-61779-310-3_19
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Online ISBN: 978-1-61779-310-3
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