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Immunohistochemistry of Human Hsp60 in Health and Disease: From Autoimmunity to Cancer

  • Francesco CappelloEmail author
  • Everly Conway de Macario
  • Giovanni Zummo
  • Alberto J. L. Macario
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 787)

Abstract

Hsp60 (also called Cpn60) is a chaperonin with essential functions for cell physiology and survival. Additionally, its involvement in the pathogenesis of a number of diseases (e.g., some autoimmune disorders and cancer) is becoming evident with new research. For example, the distribution and levels of Hsp60 in cells and tissues have been found altered in many pathologic conditions, and the significance of these alterations is being investigated in a number of laboratories. The aim of this ongoing research is to determine the meaning of these Hsp60 alterations with regard to pathogenetic mechanisms, diagnosis, classification of lesions, and assessing of prognosis and response to treatment. Hsp60 occurs in the mitochondria, i.e., its typical residence according to classic knowledge, and also in other locales, such as the cytosol, the cell membrane, the intercellular space, and biological fluids (e.g., blood and cerebrospinal fluid). Detection and quantitative determinations in all these locations are becoming essential components of laboratory pathology in clinics and research. Consequently, immunohistochemistry targeting Hsp60 is also becoming essential for pathologists and researchers interested in disorders involving this chaperonin. In this chapter, we briefly summarize some recent discoveries on the participation of Hsp60 in the pathogenesis of human diseases and describe in detail how to perform immunohistochemical reactions for detecting the chaperonin, determining its location, and measuring its levels of expression.

Key words

Hsp60 Chaperonin Hsp60 Hsp60 immunohistochemistry Hsp60 immunostaining Hsp60 in tissues Hsp60 locations Hsp60 in cancer Hsp60 and autoimmunity Molecular mimicry Hsp60 antibodies 

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Copyright information

© Springer Science+Business Media, LLC 2011

Authors and Affiliations

  • Francesco Cappello
    • 1
    Email author
  • Everly Conway de Macario
    • 2
  • Giovanni Zummo
    • 3
  • Alberto J. L. Macario
    • 2
  1. 1.Department of Experimental Biomedicine and Clinical NeuroscienceUniversity of PalermoPalermoItaly
  2. 2.Department of Microbiology and Immunology, School of MedicineUniversity of Maryland at BaltimoreBaltimoreUSA
  3. 3.Department of Experimental Biomedicine and Clinical NeurosciencesUniversity of PalermoPalermoItaly

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