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Protein Microarrays pp 237–245Cite as

Microspot Immunoassay-Based Analysis of Plasma Protein Profiles for Biomarker Discovery Strategies

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 785))

Abstract

To expedite the development of personalized medicine, new and reliable biomarkers are required to facilitate early diagnosis, to determine prognosis, predict response or resistance to different therapies, and to monitor disease progression or recurrence. Human body fluids, such as blood, present a promising resource for biomarker discovery, in every sense. Microspot immunoassays allow the simultaneous quantification of multiple analytes from a minute amount of samples in a single measurement. The experimental design of microspot immunoassays is based on antibody pairs recognizing different epitopes of the analyte. The first antibody is used to capture the analyte from the complex sample, and the second antibody is used for detection. As with traditional enzyme-linked immunosorbent assays, highly reliable and reproducible results are obtained.

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Acknowledgments

This work was supported by the German Federal Ministry for Education and Science in the framework of the Program for Medical Genome Research (grants 01GS0890 and 01GS0864), the Program for Medical Systems Biology (grant 0315396B), as well as the Helmholtz Systems Biology Initiative (SBCancer). Thanks go also to Liz Segal for her help with proofreading.

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Authors and Affiliations

  1. Division of Molecular Genome Analysis, German Cancer Research Center (DKFZ), Heidelberg, Germany

    Johanna Sonntag, Heiko Mannsperger, Anika Jöcker & Ulrike Korf

Authors
  1. Johanna Sonntag
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  2. Heiko Mannsperger
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  3. Anika Jöcker
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  4. Ulrike Korf
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Corresponding author

Correspondence to Ulrike Korf .

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Editors and Affiliations

  1. (DKFZ), Division of Molecular Genome Analysis, Deutsches Krebsforschungszentrum, Im Neuenheimer Feld 580, Heidelberg, 69120, Germany

    Ulrike Korf

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Sonntag, J., Mannsperger, H., Jöcker, A., Korf, U. (2011). Microspot Immunoassay-Based Analysis of Plasma Protein Profiles for Biomarker Discovery Strategies. In: Korf, U. (eds) Protein Microarrays. Methods in Molecular Biology, vol 785. Humana Press. https://doi.org/10.1007/978-1-61779-286-1_16

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  • DOI: https://doi.org/10.1007/978-1-61779-286-1_16

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  • Publisher Name: Humana Press

  • Print ISBN: 978-1-61779-285-4

  • Online ISBN: 978-1-61779-286-1

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