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Human Embryonic and Induced Pluripotent Stem Cells pp 351–358Cite as

Adipogenic Differentiation of Human Induced Pluripotent Stem Cells

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Abstract

Human induced pluripotent stem (iPS) cells are promising sources of disease modeling and regenerative medicine. However, differentiation properties of human iPS cells to specific lineages still remain to be fully clarified. Here, we describe a protocol for differentiating human iPS cells into adipocytes via embryoid body formation. We found that human iPS cells differentiated using this protocol exhibit lipid accumulation and the expression of adipogenic genes, suggesting that human iPS cells have adipogenic properties in vitro.

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Authors and Affiliations

  1. Department of Medicine and Clinical Science, Kyoto University Graduate School of Medicine, Kyoto, Japan

    Michio Noguchi, Masakatsu Sone, Daisuke Taura, Ken Ebihara, Kiminori Hosoda & Kazuwa Nakao

Authors
  1. Michio Noguchi
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  2. Masakatsu Sone
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  3. Daisuke Taura
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  4. Ken Ebihara
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  5. Kiminori Hosoda
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  6. Kazuwa Nakao
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Corresponding author

Correspondence to Michio Noguchi .

Editor information

Editors and Affiliations

  1. , College of Engineering, University of Arkansas, University of Arkansas 1, Fayetteville, 72701, Arkansas, USA

    Kaiming Ye

  2. , College of Engineering, University of Arkansas, University of Arkansas 1, Fayetteville, 72701, Arkansas, USA

    Sha Jin

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© 2011 Humana Press

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Noguchi, M., Sone, M., Taura, D., Ebihara, K., Hosoda, K., Nakao, K. (2011). Adipogenic Differentiation of Human Induced Pluripotent Stem Cells. In: Ye, K., Jin, S. (eds) Human Embryonic and Induced Pluripotent Stem Cells. Springer Protocols Handbooks. Humana Press. https://doi.org/10.1007/978-1-61779-267-0_25

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  • DOI: https://doi.org/10.1007/978-1-61779-267-0_25

  • Published:

  • Publisher Name: Humana Press

  • Print ISBN: 978-1-61779-266-3

  • Online ISBN: 978-1-61779-267-0

  • eBook Packages: Springer Protocols

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