Abstract
Molecular motors perform work in cells by moving in an ATP-dependent manner along filamentous tracks. In vitro, the mechanical action of such motor proteins can be investigated by attaching the molecules to surfaces in the so-called gliding or bead assays. Surface attachment protocols have to be used that do not interfere with the function of the molecule. Here, we describe a sandwich protocol that preserves functionality. The protocol can be used for a large variety of proteins, in particular kinesin motor proteins that are GFP-tagged.
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References
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Acknowledgments
The authors acknowledge the input of members of the Howard, Schäffer, and Diez labs in the development of these protocols.
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Bormuth, V., Zörgibel, F., Schäffer, E., Howard, J. (2011). Functional Surface Attachment in a Sandwich Geometry of GFP-Labeled Motor Proteins. In: Mashanov, G., Batters, C. (eds) Single Molecule Enzymology. Methods in Molecular Biology, vol 778. Humana Press. https://doi.org/10.1007/978-1-61779-261-8_2
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DOI: https://doi.org/10.1007/978-1-61779-261-8_2
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