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Analysis of Microtubule Assembly Kinetics Using Turbidimetry

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Microtubule Dynamics

Part of the book series: Methods in Molecular Biology ((MIMB,volume 777))

Abstract

Turbidity measurements are rapid and reliable methods to follow the effects of drugs, proteins, nucleotides, metals, and other factors on the assembly kinetics of tubulin into microtubules in vitro and have been in use since 1974. Improvements in spectrophotometers and software have resulted in the use of less protein, and more curves can be analyzed continuously and almost simultaneously.

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Correspondence to Felicia Gaskin .

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Gaskin, F. (2011). Analysis of Microtubule Assembly Kinetics Using Turbidimetry. In: Straube, A. (eds) Microtubule Dynamics. Methods in Molecular Biology, vol 777. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-61779-252-6_7

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  • DOI: https://doi.org/10.1007/978-1-61779-252-6_7

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-61779-251-9

  • Online ISBN: 978-1-61779-252-6

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