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Analysis of Chloroplast Movement and Relocation in Arabidopsis

  • Masamitsu Wada
  • Sam-Geun Kong
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 774)

Abstract

Chloroplast photorelocation movement is essential for the sessile plant survival and plays a role for efficient photosynthesis and avoiding photodamage of chloroplasts. There are several ways to observe or detect chloroplast movement directly or indirectly. Here, techniques for the induction of chloroplast movement and how to detect the responses, as well as various points of attention and advice for the experiments, are described.

Key words

Accumulation Arabidopsis Avoidance Blue light Chloroplast Light Microbeam Movement Movie Phototropin 

Notes

Acknowledgments

The authors thank Mr. Hidenori Tsuboi for time-lapse images of chloroplast movement induced by microbeam irradiation and Dr. Isao Uemura (Tokyo Metropolitan University) for his support on electron microscopy. This work was supported in part by Grants-in-Aid for scientific research from the Ministry of Education, Culture, Sports, Science and Technology of Japan (MEXT) (Grants 17084006 to M.W. and 21770050 to S.-G.K.) and the Japan Society for the Promotion of Science (JSPS) (Grant 20227001 to M.W.).

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Copyright information

© Springer Science+Business Media, LLC 2011

Authors and Affiliations

  1. 1.Department of BiologyKyushu UniversityFukuokaJapan

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