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Analysis of Plastid Number, Size, and Distribution in Arabidopsis Plants by Light and Fluorescence Microscopy

  • Kevin Pyke
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 774)

Abstract

Methods are described which allow one to observe chloroplasts in mesophyll cells from leaves of Arabidopsis, determine their number per cell, measure their area, and determine a value for chloroplast coverage inside mesophyll cells. Non-green plastids can also be imaged either by using staining, or by exploiting fluorescent proteins targeted to the plastid in non-green parts of the plant, such as the roots, in transgenic Arabidopsis.

Key words

Arabidopsis Chloroplast Non-green plastid Roots Fluorescent protein Protoplasts 

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Copyright information

© Springer Science+Business Media, LLC 2011

Authors and Affiliations

  1. 1.Division of Plant and Crop Sciences, School of BiosciencesUniversity of NottinghamLeicestershireUK

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